John Stones has been ruled out of England's upcoming Nations League matches.
The Inter Milan defender has suffered a thigh injury, his club have confirmed.
Stones is set to be sidelined for up to a month after straining a muscle in his left thigh.
As a result, the 32-year-old will not return to action until after the upcoming international break.
It follows tests on the issue, which Stones picked up in Monday's 5-3 win over Udinese.
The 32-year-old joined Inter on a two-year deal in July following his Manchester City exit.
Stones has since made four appearances for the Serie A giants, with the clash with Udinese representing his first start.
However, he was forced off in the 62nd minute after picking up the injury.
Thomas Tuchel is set to name his first England squad since the World Cup on Friday.
The Three Lions return to action against Spain at Wembley Stadium on September 26.
They then travel to take on the Czech Republic and Croatia as part of their Nations League campaign.
England conclude the international break on October 6 at home to the Czech Republic.
Tuchel now faces a potential defensive headache in the absence of Stones at centre-back.
The former City man featured five times at the World Cup as England finished third in North America.
As well as starting their opener against Croatia, Stones played every minute of both the quarter-final and semi-final matches.
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